Sowing the test material
Fundamental methods of sowing applicable to the clinical (material from patients) and hygienic (sanitary-microbiological control of the environment) diagnostics are: quantitative and qualitative methods. For right choice of planting method in clinical diagnosis with objective evaluation of the final result, there exists a conditional division of clinical material for sterile (blood, cerebrospinal fluid, bile, cerebrospinal fluid (CSF)), the material conventionally aseptic (discharge open wounds, urine, pick up in free or urinating catheter), i.e. material may microflora contaminated environment or adjacent habitats; material normally has its own flora (faeces, vaginal discharge, etc.). Normally sterile material is always sown qualitative method, because possession of bacteriological seeding even one colony indicates the etiological significance of the isolated microorganism (with the exclusion of errors in aseptically step collection and transportation). Also carried out a qualitative method sowing pathogenic flora. The choice of method of sowing is directly dependent on the method of collection of material, ie, if it is withdrawn surgical manipulation (lumbar puncture, etc.), the bacteriological research conducted qualitative method. Quantitative method involves serial dilutions and the use of the Gold, which is determined by seeding 1g 1ml or clinical material, expressed in number of colony- forming units (CFUs).
|
